Journal: International Journal of Molecular Medicine

Article Title: IDH2 lactylation regulates mitochondrial dysfunction injury induced by myocardial ischemia-reperfusion via the AMPK signaling pathway

doi: 10.3892/ijmm.2026.5833

Figure Lengend Snippet: SIRT3 regulates myocardial oxidative injury by delactylating IDH2 in MIRI. (A) IDH2 docked with SIRT3, SIRT4, and SIRT6 molecules, respectively. (B) The level of IDH2 lactylation detected by IP assay after overexpressing SIRT3, SIRT4, SIRT6 (n=5). (C) SIRT3 protein expression levels in MIRI detected by western blot (n=5). (D and E) The level of IDH2 lactylation detected by IP experiment and semi-quantitative analyses (n=5). (F) α-KG production under 3-TYP treatment (n=5). (G) Protein levels of p-AMPK, AMPK, DRP1, MnSOD2 and Cyt C assayed by western blotting after 3-TYP treatment, and their semi-quantitative analyses (n=5). (H and I) Representative images of DHE staining for 3-TYP treatment group (n=5). (J and K) The NADPH/NADP + ratio and CK-MB levels in the 3-TYP treatment group (n=5). These data were representative results of three repetitions at least. Data are expressed as the mean ± SD. * P<0.05, ** P<0.01 and *** P<0.001. IDH2, isocitrate dehydrogenase 2; MIRI, myocardial ischemia-reperfusion injury; IP, immunoprecipitation; 3-TYP, 3-(1H-1,2,3-Triazol-4-yl) pyridine; α-KG, α-ketoglutaric acid; p-, phosphorylated; AMPK, adenosine 5′-monophosphate-activated protein kinase; DRP1, dynamin-related protein 1; MnSOD2, manganese superoxide dismutase 2; Cyt C, cytochrome C; CK, creatine kinase; WT, wild-type; I/R, ischemia/reperfusion.

Article Snippet: Following myocardial I/R, mice were treated with either 400 mg/kg lactate (cat. no. L1750; MilliporeSigma) via intraperitoneal (i.p.) injection, or with 50 mg/kg 3-(1H-1,2,3-Triazol-4-yl) pyridine (3-TYP; cat. no. HY-108331; MedChemExpress) i.p. 24 h prior to I/R.

Techniques: Expressing, Western Blot, Staining, Immunoprecipitation